01 / Chimeric planarian analysis / ilastik
Following donor tissue through a living planarian chimera.
Worm Profiler is an end-to-end imaging pipeline for cross-strain Schmidtea polychroa transplantation experiments. It crops each 26 MP capture to the worm region so Ilastik can process it efficiently, uses pigmentation contrast to separate donor tissue, host tissue, eyes, and background, validates donor masks against hand-drawn ground truth, then measures graft area, position, shape, confidence, and change over time.